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Fig. 5 In vivo therapeutic effects of Dex@NBs-TRPC6. (A) Schematic diagram of in vivo treatment and therapy protocol. Normal <t>mouse</t> without any treat ment was dedicated as control group. (B) Albuminuria <t>(urine</t> <t>albumin-to-creatinine</t> ratio) was determined in control group, ADR + NS group and three Dex-treated ADR groups. n = 9–10 mice per group. (C) Representative kidney images of PAS staining. Upper: glomerular morphology, scale bar = 20 μm; Lower: tubule-interstitial morphology, scale bar = 50 μm. (D) Quantitative analysis of glomerulosclerosis severity. (E) Quantitative analysis of tubular injury. (F) RT-PCR analysis of mRNA expression of Kim-1. (G) RT-PCR analysis of mRNA expression of NGAL. n = 9. (H) Representative images of TEM of glomerular basement membrane. Top: scale bar = 2 μm, Bottom: scale bar = 500 nm. (I) Quantitative analysis of foot process width. n = 6 mice per group in all histo logical analysis. (J) Upper: immunofluorescence staining of WT1 expressed in nuclei of mouse podocytes, scale bar = 20 μm. Lower: immunofluorescence staining of podocin expressed along the glomerular basement membrane, scale bar = 20 μm. (K) Quantitative analysis of WT1 positive cell numbers per glomerulus, n = 6. (L) Quantitative analysis of fluorescence intensity of podocin, n = 6. Data are presented as mean ± SEM, one-way ANOVA. *p < 0.05, **p < 0.01, vs. Control group, #p < 0.05, ##p < 0.01, vs. ADR + NS group, $p < 0.05, $$p < 0.01 as indicated
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Fig. 5 In vivo therapeutic effects of Dex@NBs-TRPC6. (A) Schematic diagram of in vivo treatment and therapy protocol. Normal <t>mouse</t> without any treat ment was dedicated as control group. (B) Albuminuria <t>(urine</t> <t>albumin-to-creatinine</t> ratio) was determined in control group, ADR + NS group and three Dex-treated ADR groups. n = 9–10 mice per group. (C) Representative kidney images of PAS staining. Upper: glomerular morphology, scale bar = 20 μm; Lower: tubule-interstitial morphology, scale bar = 50 μm. (D) Quantitative analysis of glomerulosclerosis severity. (E) Quantitative analysis of tubular injury. (F) RT-PCR analysis of mRNA expression of Kim-1. (G) RT-PCR analysis of mRNA expression of NGAL. n = 9. (H) Representative images of TEM of glomerular basement membrane. Top: scale bar = 2 μm, Bottom: scale bar = 500 nm. (I) Quantitative analysis of foot process width. n = 6 mice per group in all histo logical analysis. (J) Upper: immunofluorescence staining of WT1 expressed in nuclei of mouse podocytes, scale bar = 20 μm. Lower: immunofluorescence staining of podocin expressed along the glomerular basement membrane, scale bar = 20 μm. (K) Quantitative analysis of WT1 positive cell numbers per glomerulus, n = 6. (L) Quantitative analysis of fluorescence intensity of podocin, n = 6. Data are presented as mean ± SEM, one-way ANOVA. *p < 0.05, **p < 0.01, vs. Control group, #p < 0.05, ##p < 0.01, vs. ADR + NS group, $p < 0.05, $$p < 0.01 as indicated
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Fig. 5 In vivo therapeutic effects of Dex@NBs-TRPC6. (A) Schematic diagram of in vivo treatment and therapy protocol. Normal mouse without any treat ment was dedicated as control group. (B) Albuminuria (urine albumin-to-creatinine ratio) was determined in control group, ADR + NS group and three Dex-treated ADR groups. n = 9–10 mice per group. (C) Representative kidney images of PAS staining. Upper: glomerular morphology, scale bar = 20 μm; Lower: tubule-interstitial morphology, scale bar = 50 μm. (D) Quantitative analysis of glomerulosclerosis severity. (E) Quantitative analysis of tubular injury. (F) RT-PCR analysis of mRNA expression of Kim-1. (G) RT-PCR analysis of mRNA expression of NGAL. n = 9. (H) Representative images of TEM of glomerular basement membrane. Top: scale bar = 2 μm, Bottom: scale bar = 500 nm. (I) Quantitative analysis of foot process width. n = 6 mice per group in all histo logical analysis. (J) Upper: immunofluorescence staining of WT1 expressed in nuclei of mouse podocytes, scale bar = 20 μm. Lower: immunofluorescence staining of podocin expressed along the glomerular basement membrane, scale bar = 20 μm. (K) Quantitative analysis of WT1 positive cell numbers per glomerulus, n = 6. (L) Quantitative analysis of fluorescence intensity of podocin, n = 6. Data are presented as mean ± SEM, one-way ANOVA. *p < 0.05, **p < 0.01, vs. Control group, #p < 0.05, ##p < 0.01, vs. ADR + NS group, $p < 0.05, $$p < 0.01 as indicated

Journal: Journal of nanobiotechnology

Article Title: TRPC6-targeted dexamethasone nanobubbles with ultrasound-guided theranostics for adriamycin-induced nephropathy.

doi: 10.1186/s12951-025-03487-8

Figure Lengend Snippet: Fig. 5 In vivo therapeutic effects of Dex@NBs-TRPC6. (A) Schematic diagram of in vivo treatment and therapy protocol. Normal mouse without any treat ment was dedicated as control group. (B) Albuminuria (urine albumin-to-creatinine ratio) was determined in control group, ADR + NS group and three Dex-treated ADR groups. n = 9–10 mice per group. (C) Representative kidney images of PAS staining. Upper: glomerular morphology, scale bar = 20 μm; Lower: tubule-interstitial morphology, scale bar = 50 μm. (D) Quantitative analysis of glomerulosclerosis severity. (E) Quantitative analysis of tubular injury. (F) RT-PCR analysis of mRNA expression of Kim-1. (G) RT-PCR analysis of mRNA expression of NGAL. n = 9. (H) Representative images of TEM of glomerular basement membrane. Top: scale bar = 2 μm, Bottom: scale bar = 500 nm. (I) Quantitative analysis of foot process width. n = 6 mice per group in all histo logical analysis. (J) Upper: immunofluorescence staining of WT1 expressed in nuclei of mouse podocytes, scale bar = 20 μm. Lower: immunofluorescence staining of podocin expressed along the glomerular basement membrane, scale bar = 20 μm. (K) Quantitative analysis of WT1 positive cell numbers per glomerulus, n = 6. (L) Quantitative analysis of fluorescence intensity of podocin, n = 6. Data are presented as mean ± SEM, one-way ANOVA. *p < 0.05, **p < 0.01, vs. Control group, #p < 0.05, ##p < 0.01, vs. ADR + NS group, $p < 0.05, $$p < 0.01 as indicated

Article Snippet: Mouse urine albumin concentration was measured using an ELISA kit (Bethyl Laboratories.

Techniques: In Vivo, Control, Staining, Reverse Transcription Polymerase Chain Reaction, Expressing, Membrane, Immunofluorescence, Fluorescence